Protein_Domain

Part:BBa_K2812000:Design

Designed by: Guido Oerlemans, Maxime van den Oetelaar and Mariska Brüls   Group: iGEM18_TU-Eindhoven   (2018-09-23)


Carbohydrate-binding domain from MpIBP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

DNA sequence codon optimized for E. coli.

Source

Genomic sequence from M. primoryensis. Protein sequence obtained from Guo et al. 2017. Construct synthesized de novo by IDT.

References

Guo, S., Stevens, C., Vance, T., Olijve, L., Graham, L., Campbell, R., . . . Davies, P. (2017). Structure of a 1.5-MDa adhesin that binds its Antarctic bacterium to diatoms and ice. Science Advances, 1-10.